Comparing the Karyotype of the European Domestic Goose and the Asian Goose on the Basis of the Karyotype of their Interspecific Cross-breed, Using the RBG Chromosome Staining Technique

Abstract

The aim of the research was to compare the karyotypes of two goose species: the European domestic goose and the Asian goose on the basis of the karyotype of their interspecific cross-breed, using the RBG chromosome staining technique. The karyotype standard for Anseriformes has not been determined yet. The RBG technique is considered as one of the standard methods for analysing chromosomes. It is a dynamic method. The R bands appear during the cell growth cycle in the early S phase. The formation of the characteristic band configuration for each chromosome facilitates chromosome segregation and analysis. The mitotic chromosomes for experiments were obtained from an in vitro blood lymphocyte culture and stained according to the RBG technique. The first eight largest autosome pairs and the ZW sex chromosomes were analysed. No differences were found between the band patterns of the analysed chromosomes, except for the fourth autosome pair.