Abstract
This work used computer simulation to evaluate marker-assisted selection (MAS) in recurrent selection of autogamous species. Base populations were simulated by crossing each of five inbred lines with two others. Within each cross, 400 S 0 plants were genotyped in relation to 130 marker loci spread over a fictitious genome with 50 quantitative trait loci (QTLs) randomly located. Progenies derived from such plants were taken as selection units. MAS was applied using the selection index of Lande and Thompson (1990). For most situations considered, MAS was not efficient after the first selection cycle. When considering a single cycle of selection, MAS was efficient, but strongly affected by population size and heritability. Selection of a single progeny per cross did increase linkage disequilibrium, but caused very high levels of fixation rates, which were not reduced neither with the use of 200 marker loci, nor with 20 inbred lines to form base populations.
Highlights
Recurrent selection was proposed in autogamous species early in the 1960 ́s (Khadr and Frey, 1965; Matzinger and Wernsman, 1968), and its use in many crops has increased ever since (Miller and Fehr, 1979; Payne et al, 1986; McFerson and Frey, 1991; Beaver and Kelly, 1994)
Proportion p of genetic variance explained by molecular markers was considerably high in all selective cycles (Table 1)
The average linkage disequilibrium value observed in such generations (0.160) was taken as a reference (100%)
Summary
Recurrent selection was proposed in autogamous species early in the 1960 ́s (Khadr and Frey, 1965; Matzinger and Wernsman, 1968), and its use in many crops has increased ever since (Miller and Fehr, 1979; Payne et al, 1986; McFerson and Frey, 1991; Beaver and Kelly, 1994). Methodologies to enhance its efficiency are always sought, like male sterility (Werner and Wilcox, 1990) and the use of hill plots (Pomeranke and Stuthman, 1992). Marker-assisted selection (MAS) can be another such methodology. Apuya et al, 1988), but the development of highly efficient PCRbased techniques, such as AFLP and VNTR (Kochert, 1994), has broadened the usefulness of molecular markers in such species. Regarding RFLP and RAPD markers, molecular maps are already available for a variety of crops; a detailed list was presented by Phillips and Vasil (1994)
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