Abstract
Objective: Asparagi Radix is a commonly used herbal material in Traditional Chinese Medicine. A positive phase liquid chromatography fingerprints method was developed to compare the differences between Asparagi Radix and its decoction pieces and counterfeits. Methods: The chromatographic separation was performed on a GOLD HILIC column (250 mm × 4.6 mm, 5 μm) with a gradient elution program using a mixture of acetonitrile and 0.01% phosphoric acid water as mobile phase at 220 nm wavelength, the flow rate was 0.8mL/min and the column temperature was 30°C. Results: The fingerprints of Asparagi Radix from different sources and its decoction pieces and counterfeits were determined by HPLC, and the similarity was analyzed. Eight common peaks were identified of 5 batches of Asparagi Radix from different origins, with similarity of 0.503-0.812. One common peak was identified by fingerprints of 5 batches of Asparagi Radix and 9 batches of its decoction pieces, with similarity of 0.407-0.766. Five batches of Asparagi Radix and two batches of its counterfeit were identified three common peaks, the similarity was 0.511-0.727. Three common peaks were identified by fingerprint of 9 batches of Asparagi Radix decoction pieces, with similarity of 0.507-0.793. Conclusion: By comparing the similarity of different origins of Asparagi Radix, the similarity of Asparagi Radix and its decoction pieces, the similarity of Asparagi Radix and its counterfeits, it was found that the common chemical compounds of Asparagi Radix was less, and the difference was more significant. The results indicated that the developed method could be reflect the differences in chemical composition among different sources of Asparagi Radix, its decoction pieces and its counterfeits, and served for the quality identification.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
