Abstract
The proteostasis system of animals, including various types of protein modification during the growth stage, leads to an almost incomprehensible number of possible forms of protein, and each can regulate numerous functions. In the presented work, the composition of muscle tissue protein from different portions of piglets was studied to understand the main muscle protein formation. Comparative analysis of weaned piglets' main muscle protein from l. dorsi, biceps femoris, and brachiocephalicus were analyzed using two-dimensional electrophoresis. Changes in the staining intensity of protein fractions inherent in different muscles were revealed. As part of this work, candidate groups of pig muscle proteins have been selected. Eleven protein spots were revealed for the longest muscle of the back, and seven for the biceps; the muscles of the neck are characterized by indicators of low protein fraction volume. Among the proteins found, myosin light chains, phosphoglycerate mutase, troponins, and adenylate kinase is most likely present. The obtained results of protein identification in muscle tissues, obtained during the intensive growth period, will allow a more detailed understanding of protein regulation, function, and interactions in complex biological systems, which will subsequently be significantly important for biomonitoring health and predicting farm animals productivity.
Highlights
The increase in demand for lean pork has required the selection of carcasses showing increased muscle mass
Protein spots indicated by numbers 1 and 2 in Figure 1, and presumably myosin light chains fast (MLC1f) (Murgiano et al, 2010; Yang et al, 2016) and slow (MLC1s/v), respectively, were quite well manifested in the longest muscle (Kim et al, 2017; Zou et al, 2018)
As part of this work, data were obtained on the variations in muscle protein groups in the muscles of different regions in piglets
Summary
The increase in demand for lean pork has required the selection of carcasses showing increased muscle mass. The selection for this indicator has significantly improved the meat qualities of most domestic and foreign pig breeds. It should be noted that the selection efficiency is assessed mainly by identifying genes that control beneficial traits, as well as polymorphic DNA variants in these genes, which directly affect the phenotype of the animal. In this case, genome and proteome interactions are completely ignored. Practical applications in identifying counterfeit meat products could be based on this technique (Mora, Gallego and Toldrá, 2018)
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