Comparative study of vanadium biokinetics in three species of echinoderms

Abstract

Vanadium-48 (as vanadate) was used to study the uptake, tissue distribution, depuration and food-chain transfer of vanadium through 3 species of echinoderms: the seastar Marthasterias glacialis L., the sea urchin Paracentrotus lividus Lmk. and the holothurian Holothuria forskali D.Ch.; all were collected from the littoral zone near Monaco. Uptake by all species was relativelyslow; after 3 wk exposure, isotopic equilibrium had not been reached and whole-body concentration factors ranged from 5 and 7 in the holothurian and sea urchin, respectively, to 18 in the seastar. Sixty-three to 77% of the incorporated radiotracer was associated with the body wall or test, suggesting surface sorption as the principal mechanism governing uptake from water. Stable vanadium measurements confirmed the preponderance of this element in the external hard parts of the echinoderms; however, concentration factors based on stable vanadium levels were significantly higher than those measured experimentally. Subsequent vanadium depuration rates were also species-dependent, with biological half-times for loss ranging from approximately 50 d in the sea urchin and holothurian to 123 d in the seastar. Food-chain transfer experiments indicated that seastars can assimilate and retain a large fraction of the vanadium ingested with food whereas sea urchins appear to lack this capability. The relative importance of the water and food input pathway in achieving vanadium levels in echinoderms is discussed in light of results of 48V distribution in experimental individuals and stable vanadium distribution in samples from the natural environment.