Abstract
Thunbergia laurifolia (TL) is plant widely distributed in South East Asia. TL leaves have been used as herbal infusion tea for treating fever and chemical intoxication. In this study, we examined the content of bioactive phenolic acids and flavonoids from leaves of TL in different solvent extraction. Besides, in vitro callus culture of TL was established. The rat skeletal muscle (L6) cell model was performed to investigate the glucose uptake stimulation. TL leaves extract with absolute methanol produced higher glucose uptake stimulation than those of the other solvent extracts. Interestingly, callus of TL was successful in producing high rosmarinic acid content over than intact leaves (increase up to 96-fold). The enriched rosmarinic acid callus extract significantly enhanced glucose uptake activity and elevated AMPK-α2 and GLUT4 mRNA expression. The established TL callus culture can be used as a promising source for production of biologically active phenolic acid with in vitro antidiabetic effect. Practical applications The boiled water extraction via hot infusion as herbal drinking tea could extract bioactive constituents, caffeic acid, rutin, and apigenin from Thunbergia laurifolia (TL) leaves, which enhanced glucose uptake activity. Moreover, the enriched rosmarinic acid in TL callus culture can be applied for promoting as a new source of rosmarinic acid, which exhibited antidiabetic activity.
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