Abstract
The diatom Phaeodactylum tricornutum is a marine unicellular microalga that exists under three main morphotypes: oval, fusiform, and triradiate. Previous works have demonstrated that the oval morphotype of P. tricornutum Pt3 strain presents specific metabolic features. Here, we compared the cellular organization of the main morphotypes of the diatom P. tricornutum Pt3 strain through transmission electron and advanced light microscopies. The three morphotypes share similarities including spectral characteristics of the plastid, the location of the nucleus, the organization of mitochondria around the plastid as well as the existence of both a F-actin cortex, and an intracellular network of F-actin. In contrast, compared to fusiform and triradiate cells, oval cells spontaneously release proteins more rapidly. In addition, comparison of whole transcriptomes of oval versus fusiform or triradiate cells revealed numerous differential expression of positive and negative regulators belonging to the complex dynamic secretory machinery. This study highlights the specificities occurring within the oval morphotype underlying that the oval cells secrete proteins more rapidly.
Highlights
During the last decades, knowledge regarding cell biology of eukaryotic model organisms like plants, yeast, animal cells have increased tremendously (Bezanilla, 2013; Martin, 2014; Mathur et al, 2017)
The analysis of the ultrastructure of the three morphotypes was performed by transmission electron microscopy (TEM)
The sections reveal cells surrounded by the frustule which is poorly silicified in the fusiform and triradiate cells (Figures 1A,C,D), compared to the oval cells (Figure 1B; Borowitzka and Volcani, 1978; Francius et al, 2008; Tanaka et al, 2015)
Summary
Knowledge regarding cell biology of eukaryotic model organisms like plants, yeast, animal cells have increased tremendously (Bezanilla, 2013; Martin, 2014; Mathur et al, 2017). Comparative Analysis of Pt3 Morphotypes pathways that belongs either to the plant or animal kingdoms (C4 photosynthetic pathway and urea cycle, for example) (Butler et al, 2020) It is a photoautotrophic organism for which molecular tools as well as transformation methods have been developed (Apt et al, 1996; Zaslavskaia et al, 2000; Niu et al, 2012; Miyahara et al, 2013; Zhang and Hu, 2014; Karas et al, 2015). Metabolic engineering strategies could be envisioned and implemented as exemplified for the production of high-value plant triterpenoid production (D’Adamo et al, 2019) and increase of lipid accumulation (Zou et al, 2018)
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