Abstract
The genus Kangiella has recently been proposed within the family Kangiellaceae, belonging to order Oceanospirillales. Here, we report the complete genome sequence of a novel strain, Kangiella profundi FT102, which is the only Kangiella species isolated from a deep sea sediment sample. Furthermore, gaps in the publicly available genome scaffold of K. aquimarina DSM 16071 (NCBI Reference Sequence: NZ_ARFE00000000.1) were also filled using polymerase chain reaction (PCR) and Sanger sequencing. A comparative genomic analysis of five Kangiella and 18 non-Kangiella strains revealed insights into their metabolic potential. It was shown that low genomic redundancy and Kangiella-lineage-specific gene loss are the key reasons behind the genome reduction in Kangiella compared to that in any other free-living Oceanospirillales strain. The occurrence of relatively diverse and more frequent extracellular protease-coding genes along with the incomplete carbohydrate metabolic pathways in the genome suggests that Kangiella has high extracellular protein degradation potential. Growth of Kangiella strains has been observed using amino acids as the only carbon and nitrogen source and tends to increase with additional tryptone. Here, we propose that extracellular protein degradation and amino acid utilization are significant and prominent features of Kangiella. Our study provides more insight into the genomic traits and proteolytic metabolic capabilities of Kangiella.
Highlights
The ocean covers 71% of the earth’s surface and is regarded as the largest habitat for life on the planet Earth
The complete genome sequence of K. profundi FT102 is composed of a circular chromosome of 2,653,010 bp with 43.81% GC content (Figure 1)
Our results showed a similar mechanism of genomic reduction, including de-redundancy and genus-specific loss of orthologs, in genus Kangiella compared to other nonKangiellaceae Oceanospirillales
Summary
The ocean covers 71% of the earth’s surface and is regarded as the largest habitat for life on the planet Earth. Marine microorganisms are known to play an essential role in energy conservation and biogeochemical cycling in the oceans. Oceanospirillales is an order of proteobacteria with seven families of heterotrophic marine bacteria that are usually associated with oil spills and are known to be involved in xylan and hydrocarbon utilization (Choi et al, 2012; Cao et al, 2014). A new family named Kangiellaceae has been proposed within the order Oceanospirillales based on phylogenetic, Extracellular-Protein Degradation Potential in Kangiella chemotaxonomic and physiological characteristics; this family comprises three genera: Kangiella, Aliikangiella, and Pleionea (Wang et al, 2015). The Kangiella genus was reclassified within Kangiellaceae instead of Alcanivoracaceae based on 16S rRNA gene phylogeny (Wang et al, 2015), taxonomic signatures at the genomic level are still needed
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