Abstract

Four random primers was utilized for detecting genetic diversity of five mustard varieties as the detection of genetic diversity is essential for proper use in the genetic improvement of the mustard. A total of 20 bands were scored corresponding to an average of five bands per primer. Level of genetic polyphorphism was in the range of 0 to 66.66%. A dendrogram constructed using the unweighted pair group method with arithmetic averages (UPGMA) revealed the maximum similarity of variety Kanti with Varuna (similarity indices 0.9677) while distantly related varieties were Basanti and Rohini. The randomly amplified polymorphic DNA (RAPD) cluster pattern showed two major clusters namely cluster-I and cluster -II. Cluster -I includes Kanti and Varuna and cluster -II includes Rohini and Urvashi. The variety Basanti occupies a distinct place as revealed in the dendrogram. Keywords: Mustard, Genetic diversity, RAPD, UPGMA.

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