Abstract
ABSTRACTThree serological methods used in the diagnosis of bovine brucellosis were conducted to assess the diagnostic sensitivity of the tests in brucellosis-infected herds. A total of 487 unvaccinated bovine serum samples collected from infected herds in the southeast region of Turkey having history of abortion with a known bacteriological status for the last three years were tested for anti-Brucella antibodies detection using the Rose Bengal plate test (RBPT), the complement fixation test (CFT) and indirect enzyme-linked immunosorbent assay (i-ELISA). A protein A/G conjugate was used in i-ELISA in order to reduce background reactivity and to use the test in multispecies if required. The serum samples were found as positive in 74.3%, 83.7% and 81.3% when we employed the CFT, i-ELISA and RBPT, respectively. i-ELISA could be used as a single test or combined with the RBPT for serologic diagnosis for brucellosis in infected herds.
Highlights
Brucellosis is a common zoonotic disease that has important veterinary and public health concerns and economical impact
A total of 487 unvaccinated bovine serum samples collected from infected herds in the southeast region of Turkey having history of abortion with a known bacteriological status for the last three years were tested for anti-Brucella antibodies detection using the Rose Bengal plate test (RBPT), the complement fixation test (CFT) and indirect enzyme-linked immunosorbent assay (i-ELISA)
Several serological tests can be used for the diagnosis of bovine brucellosis, the Rose Bengal plate test (RBPT) and the complement fixation test (CFT) are the official tests currently used for serologic diagnosis of the disease
Summary
Brucellosis is a common zoonotic disease that has important veterinary and public health concerns and economical impact. Sera from different stages of infection may not give positive results and there is currently no single serological test to detect all stages of infection. For this reason, a combination of screening and confirmatory test is generally used in order to detect infection status more accurately (Nielsen 2002; McGiven et al 2003). Anticomplementary activity needs heat inactivated serum samples and prozone phenomenon are the other limitations It requires good laboratory facilities and well-trained personnel to accurately perform the test and to maintain its reagents. Different i-ELISA techniques were the results of different antigens used and conjugates chosen for the test (Sutherland et al 1986; Wright et al 1990; Jacques et al 1998; Garcia-Bocanegra et al 2014; O’Grady et al 2014)
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