Comparative Evaluation of Freshly Prepared Versus Freeze-Dried Human Amniotic Membrane in Intraoral Soft Tissue Defects

Abstract

Aim: The aim of the study is to evaluate the efficacy in treatment outcome while using freshly prepared human amniotic membrane against freeze dried human amniotic membrane in intraoral soft tissue defects. Method: This prospective study included 20 patients having postsurgical soft tissue defect requiring primary wound coverage with human amniotic membrane. Patients were divided randomly into 2 groups. In group A, freshly prepared human amniotic membrane was placed, while in group B, freeze-dried human amniotic membrane was placed. Patients were evaluated on the basis of following parameters: postoperative pain, swelling, rate of epithelialization, and interincisal distance over a period of 6 months. Result: The results showed no significant difference in the mean of the pain between both the groups except on the first postoperative day. No significant difference in terms of reduction in swelling and increase in interincisal distance postoperatively was found at any point while using either fresh or freeze-dried human amniotic membrane. Significant difference was found in the mean rate of epithelialization between the groups on the 7th and the 21st postoperative days. Conclusion: Thus, we conclude that freshly prepared human amniotic membrane in comparison to the freeze-dried human amniotic membrane accelerates epithelialization and healing when used in the repair of postsurgical oral mucosal defects.