Abstract
Premature ovarian failure (POF) is a common consequence for woman after treatment of oncological diseases. Nowadays the actual task is to find new methods to restore and preserve the ovarian function in women after chemotherapy (CT).The aim of the research was to compare the therapeutic effects of cryoextract of placenta (CP) and mesenchymal stem cells of adipose tissue (MSCs AT) in restoring the sexual function in POF after CT in the experiment.Materials and methods: POF was modeled on female BALB \ c mice. Animals were divided into 4 groups of 15 females with a weight of 20.1 ± 1.1 g with a regular estrous cycle: 1 – intact group, 2 - group with modeled POF, 3 - group with modeled POF and treated with CP (0.01 g in / m 1 p / day for 5 days). 4-group with modeled POF and treated with MSCs AT (100,000 cells intraperitoneally at once). We studied the weight, estrous cycle, sexual activity, morphological changes in the reproductive organs in animals.Results and discussion: After CT weight was reliably reduced in all mice, the estrous cycle became monophasic, there were no vaginal plugs. In the 2nd group of animals, without treatment, weight and activity were restored at 6 weeks after CT, the estrus cycle - after 5 weeks in 40% of animals, sexual activity in 6 weeks in 30% of mice. In 3 group of animals, weight and activity were restored after 3 weeks, estrous cycle in 70% animals after 4 weeks, sexual activity - in 5 weeks. In 4 group of animals treated with MSCs AT, weight and activity were restored after 3 weeks, the estrous cycle in 85% mice after 4 weeks, sexual activity - after 5 weeks. Histological examination indicated: in 2 group of animals ovaries were reduced in size and structural elements in them were absence. In uteri there were atrophic changes. In groups of animals treated with CP and MSCs AT, there were lack of typical elements in ovaries, separate follicle-like structures were observed, the structure of uteri was close to intact.Conclusions: In the experiment KP and MSCs AT efficacy when treating the POF, induced by CT.
Highlights
Premature ovarian failure (POF) is a common consequence for woman after treatment of oncological diseases
Разработанный в эксперименте метод коррекции Преждевременная недостаточность яичников (ПНЯ), может использоваться для восстановления репродуктивного здоровья женщин с ПНЯ после ХТ
In groups of animals treated with CP and MSCs AT, there were lack of typical elements in ovaries, separate follicle-like structures were observed, the structure of uteri was close to intact
Summary
ПЛАЦЕНТЫ И МЕЗЕНХИМАЛЬНЫХ СТВОЛОВЫХ КЛЕТОК ЖИРОВОЙ ТКАНИ В ЛЕЧЕНИИ ПРЕЖДЕВРЕМЕННОЙ НЕДОСТАТОЧНОСТИ ЯИЧНИКОВ, ВЫЗВАННОЙ ХИМИОТЕРАПИЕЙ, В ЭКСПЕРИМЕНТЕ. Преждевременная недостаточность яичников (ПНЯ) является частым последствием лечения онкологических заболеваний. Цель исследования: сравнить терапевтические эффекты криоэкстракта плаценты (КП) и мезенхимальных стволовых клеток жировой ткани (МСК ЖТ) в восстановлении половой функции при овариальной недостаточности после ХТ в эксперименте. Во 2 группе животных без лечения вес и активность восстанавливались через 6 недель после ХТ, эстральный цикл через 5 недель у 40%, половая активность — через 6 недель у 30% животных. В 3 группе животных, вес и активность восстанавливались через 3 недели, эстральный цикл у 70% —через 4 недели, половая активность — через 5 недель. В 4 группе животных, после применения МСК ЖТ, вес и активность восстанавливались через 3 недели, эстральный цикл у 85% — через 4 недели, половая активность — через 5 недель. Ключевые слова: Осложнения после химиотерапии, преждевременная недостаточность яичников, криоэкстракт плаценты, мезенхимальные стволовые клетки из жировой ткани. Что жировая ткань в значительных количествах (до 300 мл и более) может быть получена под местной анестезией при сравнительно малоболезненной косметической липосакции, липоаспирации подкожного жира или путем эксцизии жировых отложений, эта ткань может явиться альтернативным костному мозгу источником МСК для трансплантации и тканевой инженерии [5]
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