Abstract
Construction of a microarray based on the genome of Xanthomonas campestris pv.campestris (Xcc), and its use to analyse 18 other virulent Xcc strains, revealed insights into the genetic diversity and determinants of host specificity of Xcc strains.
Highlights
Xanthomonas campestris pathovar campestris (Xcc) is the causal agent of black rot disease of crucifers worldwide
Several attractive divergent genetic determinants related to pathogenicity uncovered by array-based comparative genome hybridization (aCGH) analyses were further functionally characterized, enabling the discovery of avr genes affecting Xcc host specificity and the type IV secretion system (T4SS) that are not involved in symptom production by Xcc
Most of the 27 Xanthomonas variable genomic region (XVR) possess DNA sequences associated with integrase genes or mobile elements and with lower GC content and higher δ* value compared to Xcc regular genomic characteristics, implying that these DNA sequences may have been acquired through horizontal gene transfer [53,72]
Summary
Xanthomonas campestris pathovar campestris (Xcc) is the causal agent of black rot disease of crucifers worldwide. Xanthomonas campestris pathovar campestris (Xcc) is the causal agent of black rot disease, one of the most destructive diseases of cruciferous plants worldwide [1]. This pathogen infects almost all the members of the crucifer family (Brassicaceae), including important vegetables such as broccoli, cabbage, cauliflower, mustard, radish, and the major oil crop rape, as well as the model plant Arabidopsis thaliana. Genome annotation predicted that Xcc possesses at least eight genes that show sequence homology to the known avr genes discovered from other bacteria [20,21] Mutagenesis analysis of these eight avr-homologous genes detected avirulence activity for only avrXccFM [22]
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