Abstract
Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-tolerance identification in cotton.
Highlights
Soil salinization is widely distributed among the northwest, northeast, and coastal regions of China
An immobilized pH gradient (IPG) strip and the isoelectric focusing (IEF)/sodium dodecyl sulfate (SDS)-PAGE technique were utilized to profile the proteomic changes in the salt-tolerant and salt-sensitive varieties Zhong 07 and Zhong s9612, respectively, in response to 24 h salt stress
The results showed that the number and type of proteins were roughly the same between the treatment and control groups, with only a fraction of proteins showing significant changes
Summary
Soil salinization is widely distributed among the northwest, northeast, and coastal regions of China. Salt stress can strongly affect crop yield, protein synthesis, photosynthesis, and energy metabolism [1]. An increase in the salt concentration in soil can significantly impede the normal growth of cotton (Gossypium hirsutum L.) seedlings and result in physiological yield loss and death [2] [3]. Salt stress in soil has become one of the key abiotic factors threatening cotton seedling development and production. Cotton is one of the species with strong salt tolerance and is a pioneering crop in saline land. Previous studies have discussed the effects of salt on photosynthesis [4]-[7], antioxidants [8] [9], seed germination [10] [11], and cellular structure [12]. Cotton has been found to perceive and respond to various adversities, including salt stress
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