Abstract
An in-depth study of the oxidative homeostasis state into cell suspensions that contain hematopoietic stem cells is one of the key points for understanding ways to improve technologies for long-term storage of this material. Compounds with antioxidant action are considered promising additional cryoprotectants. Intensification of lipid peroxidation processes is one of the main factors causing disturbances in the barrier properties of cell membranes. Comparative analysis of changes in lipid peroxidation parameters during the cryopreservation-deconservation cycle showed that antioxidants-membrane protectors with different mechanisms of action (B-complex vitamins; α-lipoic acid, thiazotic acid morpholinium salt, 2-ethyl-6-methyl-3-hydroxy pyridine succinate) have similar features of a positive effect on the oxidative status of umbilical cord blood nuclear cell suspensions during cryopreservation. However, 2-ethyl-6-methyl-3-hydroxy pyridine succinate has a statistically significant advantage over α-lipoic acid and thiazotic acid morpholinium salt in terms of the conjugate formation dynamics during phospholipid peroxidation, which can be associated with its direct antioxidant effect.
Highlights
In the last 25 years, human umbilical cord blood (UCB) has become widespread as a source of hematopoietic stem cells [1]
Four groups of UCB nuclear cells (NCs) suspensions were compared in terms of the antioxidantmembraneprotective compounds (AOM) efficiency
The drugs were used in one concentration, which was determined as the best in previous studies on cryopreservation [12]
Summary
Kalynychenko Tetiana, Doctor of Medical Sciences, PhD, Senior Researcher, Head of Laboratory of Hemopoietic Cell Cryopreservation. State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine", Kiyv, Ukraine, ORCID ID: http://orcid.org/0000-0002-4905-3256 Anoshyna Militina, PhD, Senior Researcher, Leading Researcher, Head of Biochemistry Group. State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine", Kiyv, Ukraine, ORCID ID: http://orcid.org/0000-0001-6001-8016 Balan Valentyna, Researcher of Laboratory of Immunogenetics. State Institution “National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine, ORCID ID: http://orcid.org/0000-0001-9663-7898 Parubets Lidiia, Researcher of Laboratory of Hemopoietic Cell Cryopreservation. State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine", Kiyv, Ukraine, ORCID ID: https://orcid.org/0000-0003-3418-1316 Yagovdik Maryna, PhD, Senior Researcher of Biochemistry Group. KEYWORDS cryopreservation, umbilical cord blood, nuclear cells, lipid peroxidation, antioxidants
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