Abstract
Three genomic clones (gb/*4.2, gb/f4.6, gb/t4.9) have been isolated from a barley [Hordeum vulgare L.) cv. Igri genomic library using a cDNA clone of the low temperature responsive gene b/r4.1 as the probe. The genomic clones are closely related and from sequence homology are believed to be three further members of a barley gene family encoding lipid transfer proteins. One clone, gb/t4.9, corresponds to a second cDNA clone (Jb/f4.9). Northern blot analysis revealed that the three genes reported here are induced by low temperature and drought; however, the relative level of response to these stimuli was shown to differ between the genes. Further, the developmental response of gb/?4.2 is different from that found for other members of the gene family. The degree of low temperature induction of the blt4 gene family appears to be cultivar-dependent. Comparative analysis of the putative promoter regions of the genomic clones indicates three regions that show over 80 sequence conservation between gb/f4.9 and gb/f4.6. Also identified are putative cis acting elements previously identified in ABA responsive genes and low temperature inducible genes. The possible functional significance of the conserved regions and elements is discussed in relation to the expression of the blt4 gene family.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
