Abstract
Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical area for its action against various diseases, as is the case of cancer or heart problems. The investigation was conducted to evaluate seven non-conventional yeasts - Debaryomyces hansenii PYC ISA 1510, Debaryomyces hansenii PYC 2968, Candida parapsilosis, Candida utilis, Pichia pastoris, Pichia kluyveri, Issatchenkia terricola - as producers of the enzyme tannase. This was done in mediums of fermentation with tannic acid (1-3%) and YPD without tannic acid, to determine the activity of the enzyme by two spectrophotometric methods, the first being with rhodamine and the second with methyl gallate, to detect the presence of gallic acid as a product of the hydrolysis of tannic acid. The main results showed that the evaluated non-conventional yeasts all presented activity with the Tannase enzyme, obtaining better detection results with the rhodamine technique for the stability of the formed complex, which gives more precision of the obtained data.
Highlights
Ellagic acid is a polyphenolic compound obtained by the degradation of ellagitannins present in various fruits and plants, which has a lot of applications in various areas such as nutraceutical, pharmaceutical, cosmetic and food industry (Vázquez-Flores et al, 2012)
Zhong et al (2004) cloned and expressed the tannase gene from A. oryzae in the yeast Pichia pastoris, and Shi et al (2005) found tannase activity in the C. utilis strain. This investigation seeks to expand the information about the production of tannase in non-conventional yeast strains that have not been reported in the literature, pointing out that these strains have not been genetically modified
There was a decrease of cellular concentration while the concentration of tannic acid increased, these dates are found within the reported values for non-conventional yeast like Candida shehatae, Candida guilliermondii and Saccharomyces kluyvery, with average growth rates of 0.32 ± 0.01 h-1, 0.30 ± 0.07 h-1 and 0.19 ± 0.02 h-1, respectively, (González-Hernández et al, 2015)
Summary
Ellagic acid is a polyphenolic compound obtained by the degradation of ellagitannins present in various fruits and plants, which has a lot of applications in various areas such as nutraceutical, pharmaceutical, cosmetic and food industry (Vázquez-Flores et al, 2012). The use of fungi entails an increase in the amount of substrate required for their growth and a longer time for fermentation, which in turn has an impact on the energy costs For these disadvantages, the investigation is developing alternatives to improve said process, using non-conventional yeasts as microorganisms that produce the enzymes of interest, reducing fermentation time, costs of maintenance and adaptation of microorganisms. Zhong et al (2004) cloned and expressed the tannase gene from A. oryzae in the yeast Pichia pastoris, and Shi et al (2005) found tannase activity in the C. utilis strain This investigation seeks to expand the information about the production of tannase in non-conventional yeast strains that have not been reported in the literature, pointing out that these strains have not been genetically modified.
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