Abstract
Common Determinants of Single Channel Conductance within the Large Cytoplasmic Loop of 5-Hydroxytryptamine Type 3 and α4β2 Nicotinic Acetylcholine Receptors
Highlights
Introduction of MA0Ј Residues of nicotinic acetylcholine receptors (nAChRs) into the 5-HT3A Subunit Influences ␥—The ␥ of neuronal nicotinic receptors (e.g. ␣7 and ␣42) is much greater than that of the 5-HT3A receptor
Given that the much higher ␥ of the heteromeric 5-HT3A/5-HT3B receptor versus the homomeric 5-HT3A receptor is due, at least in part, to the nature of MA residues and the MA 0Ј residue in particular, we investigated the influence of the residues that occupy homologous MA 0Ј positions in the nAChR ␣7(Glu), 2(Gln), and ␣4(Phe) subunits on the ␥ of the 5-HT3A receptor
Given the influence of the nicotinic MA stretch Ϫ4Ј, 0Ј, and 4Ј residues on the ␥ of the 5-HT3A receptor, we examined whether the identities of the equivalent residues in nAChR subunits contribute to the ␥ of the nACh receptors
Summary
DNA Constructs and Transient Transfection of Subunit cDNAs— cDNAs encoding rat wild-type (WT) nAChR ␣4 and 2 subunits Transfected cells were routinely cultured at 37 °C for 24 –72 h before use, in the case of nicotinic receptor constructs incubation temperature was reduced to 29 °C overnight prior to recording in order to enhance cell surface expression [12].
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