Abstract
Combined impedance and extracellular field potential recordings on iPS cardiomyocytes
Highlights
Human induced pluripotent stem cell-derived cardiomyocytes have a great potential for cardiovascular research and predictive in-vitro cardiac safety screening when it comes to early detection of arrhythmic compounds
In accordance with the Comprehensive In Vitro Proarrhythmia Assay (CiPA) guidelines, standard reference compounds were tested on iPS-derived cardiomyocytes
We have shown combined impedance and extracellular field potential (EFP) measurements using a commercially available platform (CardioExcyte 96) can be made reliably from a number of different hiPSCCMs including Cor.4U (Axiogenesis AG), iCell Cardiomyocytes (Cellular Dynamics International), Pluricytes (Pluriomics) and Cellartis® hiPS-CM (Takara Bio Europe AB)
Summary
The CardioExcyte 96 system provides a non-invasive, label-free, high temporal resolution approach for safety screening on iPSCMs. It is a hybrid screening instrument that combines impedance with MEA-like extracellular field potential (EFP) recordings. It is a hybrid screening instrument that combines impedance with MEA-like extracellular field potential (EFP) recordings It can be either used in an incubator or, by utilizing the dedicated incubation system, directly on a lab bench. The system is capable of electrically pacing the cells, which allows for screening of cells which beat with individual frequencies and, in addition, investigations of frequency dependent compound inhibition
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