Abstract
A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.
Highlights
Rotaviruses are major causes of severe, potentially life-threatening gastroenteritis in young children [1]
This collection of recombinant rotaviruses provides a foundation for establishing fluorescence-based live-cell imaging methods for determining titers of the virus and monitoring spread of the virus, characterizing neutralizing antibodies and antiviral agents, and analyzing viral gene expression during the replication cycle
The recombinant rotavirus strains in this collection are available to laboratories operating with biosafety level 2 (BSL2) certification that have received institutional approval to receive and perform experiments with rotavirus
Summary
Rotaviruses are major causes of severe, potentially life-threatening gastroenteritis in young children [1]. This collection of recombinant rotaviruses provides a foundation for establishing fluorescence-based live-cell imaging methods for determining titers of the virus and monitoring spread of the virus, characterizing neutralizing antibodies and antiviral agents, and analyzing viral gene expression during the replication cycle.
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