Abstract
Pepsin solubilized collagen-carboxymethyl dextran (PSC-CMD) conjugate was prepared to improve the function of collagen by using cyanogen bromide. The conjugation between PSC and CMD was confirmed by SDS-PAGE, isoelcctric focusing and size-exclusion chromatography. The molar ratio of the conjugate was PSC:CMD=1:2.3. The isoelectric point was shifted from 9.0 to 5.5 by conjugation. Denaturation temperature of the conjugate molecule was 42.2°C, which is 1.9°C higher than that of PSC. The PSC-CMD conjugate maintained self-assembling ability but showed a very slow aggregation as compared with that of PSC. Reconstructed matrix of the conjugate had lower denaturation temperature and random molecular rearrangement structure as compared with PSC matrix. The platelet aggregation inducing ability, analyzed by spectroscopy disappeared.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
