Abstract
Nowadays, coffee beans are almost exclusively used for the preparation of the beverage. The sustainability of coffee production can be achieved introducing new applications for the valorization of coffee by-products. Coffee silverskin is the by-product generated during roasting, and because of its powerful antioxidant capacity, coffee silverskin aqueous extract (CSE) may be used for other applications, such as antiaging cosmetics and dermaceutics. This study aims to contribute to the coffee sector’s sustainability through the application of CSE to preserve skin health. Preclinical data regarding the antiaging properties of CSE employing human keratinocytes and Caenorhabditis elegans are collected during the present study. Accelerated aging was induced by tert-butyl hydroperoxide (t-BOOH) in HaCaT cells and by ultraviolet radiation C (UVC) in C. elegans. Results suggest that the tested concentrations of coffee extracts were not cytotoxic, and CSE 1 mg/mL gave resistance to skin cells when oxidative damage was induced by t-BOOH. On the other hand, nematodes treated with CSE (1 mg/mL) showed a significant increased longevity compared to those cultured on a standard diet. In conclusion, our results support the antiaging properties of the CSE and its great potential for improving skin health due to its antioxidant character associated with phenols among other bioactive compounds present in the botanical material.
Highlights
Oxidative stress is a major cause of skin accelerated aging and diseases, which is defined as the imbalance between reactive oxygen species (ROS) and antioxidants
No significant decrease (p > 0.05) of absorbance was observed after incubation of the compounds when cell viability was measured (Figure S2, Supplementary Materials). These results suggest that coffee silverskin aqueous extract (CSE), chlorogenic acid (CGA), caffeine and vitamin C at the concentrations tested in this investigation have no adverse effects on the viability of HaCaT cells
We provide scientific evidence with regard to the antioxidant protective effects of CSE in human skin cells and in vivo using C. elegans, an experimental model
Summary
Oxidative stress is a major cause of skin accelerated aging and diseases, which is defined as the imbalance between reactive oxygen species (ROS) and antioxidants. When cells are subjected to excessive levels of ROS or as a result of antioxidant depletion, oxidative stress occurs [1]. ROS can be generated by exogenous sources (UV radiation or chemical agents) and cause DNA, protein and lipid damage. This can lead to skin diseases, such as dermatitis, sunburn, acne, eczema, Molecules 2016, 21, 721; doi:10.3390/molecules21060721 www.mdpi.com/journal/molecules vasculitis, psoriasis andand cancer [3]
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