Abstract
The main aim of this work was to evaluate the efficiency of producing caproic acid and other volatile fatty acids using a co-digestion between cheese whey and sewage sludge in a continuous reactor. The effect of two different feeding regimes (one and two per day) and three hydraulic retention times (HRT) (15, 10 and 6 days) on the organic acids production were studied. The optimal conditions for the process were 10 days HRT, 2 feeding cycles per day, reaching a maximum degree of acidification of 44%. Under these conditions, the most abundant organic acid was caproic acid. The analysis of the microbial community dynamics in the reactor during the HRT changes revealed a microbiome enriched in organisms involved in caproic acid production. Additionally, the production of polyhydroxyalkanoates using the organic acids stream as feeding was verified in a fed-batch experiment obtaining a copolymer formed by hydroxybutyrate, hydroxyvalerate and hydroxyhexanoate.
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