Clotting factor activity in cryoprecipitates and supernatant plasma prepared from blood collected into ACD, ACD-adenine, CPD, and CPD-adenine and from plasma collected by plasmapheresis.

Abstract

Adenine has been used to prolong the survival of stored erythrocytes, and thus extend the storage period of blood. It seemed desirable to investigate the effect of this additive on the procoagulants of plasma that full use of cryoprecipitates and other plasma fractions could be made from blood drawn into an adenine‐enriched anticoagulant. Eight units of whole blood were drawn into each of four anticoagulants: ACD, ACD‐adenine, CPD, and CPD‐adenine. Cryoprecipitates were prepared from each unit of fresh plasma according to a modification of Pool's method. Assays for fibrinogen, prothrombin, and factors V, VII, VIII, IX, and X were performed on the cryoprecipitates and on the supernatant plasma drawn off the cryoprecipitates. Adenine did not alter the expected yield of factor VIII (AHF) in the cryoprecipitate. There was slight to moderate loss of factor V and fibrinogen, respectively, in the supernatant plasma, but prothrombin and factors VII, IX, and X were unaffected by the procedure. Assays also revealed good AHF activity in each unit of plasma from a double plasmapheresis; therefore, both units are satisfactory for use in preparing cryoprecipitates.