Abstract
Cloning, Structural Characterization, and Phylogenetic Analysis of Flower MADS-Box Genes from Crocus (Crocus sativus L.)
Highlights
Tsaftaris et al.: Crocus flower MADS-box genesTheScientificWorldJOURNAL (2007) 7, 1047–1062Flowering plants represent one of the most successful and diverse groups of organisms on the planet, with more than 250,000 extant species in the wild and many more varieties generated by horticulturists through hybridization and other breeding efforts
We have cloned, characterized, and studied the expression of all types of MADS-box genes from crocus involved in flower formation
We have developed a modification of the RCA-RACE method named familyRCA-RACE that allows the simultaneous isolation of members of a family of homologous genes in one RCA-RACE reaction by using degenerate primers corresponding to a conserved amino acid domain in antisense orientation
Summary
Flowering plants represent one of the most successful and diverse groups of organisms on the planet, with more than 250,000 extant species in the wild and many more varieties generated by horticulturists through hybridization and other breeding efforts. The ABC model holds that the overlapping domains of three classes of gene activity, referred to as A, B, and C, produce a combinatorial code that determines floral organ identity in successive whorls of the developing flower. Understanding flower development in crocus could reveal ways to increase yield and lower production costs since flowers and, isolated stigmas comprise the valuable commercial part of the plant With this goal, we have cloned, characterized, and studied the expression of all types of MADS-box genes from crocus involved in flower formation.
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