Abstract
Cold-adapted alginate lyases have unique advantages for alginate oligosaccharide (AOS) preparation and brown seaweed processing. Robust and cold-adapted alginate lyases are urgently needed for industrial applications. In this study, a cold-adapted alginate lyase-producing strain Vibrio sp. W2 was screened. Then, the gene ALYW201 was cloned from Vibrio sp. W2 and expressed in a food-grade host, Yarrowia lipolytica. The secreted Alyw201 showed the activity of 64.2 U/mL, with a molecular weight of approximate 38.0 kDa, and a specific activity of 876.4 U/mg. Alyw201 performed the highest activity at 30 °C, and more than 80% activity at 25–40 °C. Furthermore, more than 70% of the activity was obtained in a broad pH range of 5.0–10.0. Alyw201 was also NaCl-independent and salt-tolerant. The degraded product was that of the oligosaccharides of DP (Degree of polymerization) 2–6. Due to its robustness and its unique pH-stable property, Alyw201 can be an efficient tool for industrial production.
Highlights
Brown seaweed (Phaeophyceae) is considered one of the most promising bioresources due to its high growth rate and huge biomass [1]
Robust and cold-adapted alginate lyases are urgently needed for industrial applications
The results show that Alyw201 of the PL7the family, such conserved as “RT/SELRE,”
Summary
Brown seaweed (Phaeophyceae) is considered one of the most promising bioresources due to its high growth rate and huge biomass [1] Multiple bioactive substances, such as alginate, laminarin, fucoidan, fucoxanthin, mannitol, polyphenol, and phytohormones, are abundant in brown seaweed and are applied in different industries [2,3]. Industrial enzymes generally require thermostability, several advantages can advantages can be achieved when the hydrolysis was performed during the cultivation of a be achieved when the hydrolysis was performed during the cultivation of a recombinant strain with recombinant strain with cold-adapted alginate lyases. Robust and cold-adapted alginate lyases are urgently needed for industrial applications. The secreted alginate lyase Alyw201 showed obvious obvious robustness, excellent pH stability, and NaCl-independent and salt-tolerant properties.
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