Abstract
A rapid increase in ecdysteroid hormone synthesis results when the insect prothoracic gland is stimulated with prothoracicotropic hormone (PTTH), a brain neuropeptide hormone. PTTH also stimulates the specific synthesis of several proteins, one of which is a β tubulin. To further understand the possible roles of β tubulin in the prothoracic gland, β tubulin cDNA clones were isolated from a tobacco hornworm ( Manduca sexta) gland cDNA library. Sequence analysis indicated that these clones were assignable to the β1 tubulin isoform. Gland β1 tubulin mRNA levels during the last larval instar and early pupal-adult development exhibited peaks that coincided with peaks in ecdysteroid synthesis. Manipulations of the glands hormonal milieu showed that β1 tubulin mRNA levels respond to 20 hydroxyecdysone and PTTH. The data also support our earlier proposal that the prothoracic gland β tubulin gene is ubiquitously expressed but exhibits tissue- and developmental-specific regulation of transcription and translation.
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