Abstract
Plant vacuolar Na+/H+ antiporter genes play significant roles in salt tolerance. However, the roles of the chrysanthemum vacuolar Na+/H+ antiporter genes in salt stress response remain obscure. In this study, we isolated and characterized a novel vacuolar Na+/H+ antiporter gene DgNHX1 from chrysanthemum. The DgNHX1 sequence contained 1920 bp with a complete open reading frame of 1533 bp encoding a putative protein of 510 amino acids with a predicted protein molecular weight of 56.3 kDa. DgNHX1 was predicted containing nine transmembrane domains. Its expression in the chrysanthemum was up-regulated by salt stress, but not by abscisic acid (ABA). To assess roles of DgNHX1 in plant salt stress responses, we performed gain-of-function experiment. The DgNHX1-overexpression tobacco plants showed significant salt tolerance than the wild type (WT). The transgenic lines exhibited more accumulation of Na+ and K+ under salt stress. These findings suggest that DgNHX1 plays a positive regulatory role in salt stress response.
Highlights
Salinity is one kind of environmental stress affecting plant growth and productivity worldwide
Many studies have been reported to improve salt stress resistance of plants by over-expression of vacuolar Na+/H+ antiporter genes such as AtNHX1, GhNHX1, OsNHX1, and EgNHX1 etc [3,4,6,7]. All these results suggest that vacuolar Na+/H+ antiporter genes play significant roles in salt tolerance
The DgNHX1 was structurally similar to OsNHX1, which was isolated from Oryza sativa under salt stress [6], and GhNHX1, which was isolated from Gossypium hirsutum under salt stress [7]
Summary
Salinity is one kind of environmental stress affecting plant growth and productivity worldwide. Plant can initiate an array of morphological, physiological, and biochemical adaptations to salt stress In these adaptations, many salt stress-related genes are induced [1,2]. Many studies have been reported to improve salt stress resistance of plants by over-expression of vacuolar Na+/H+ antiporter genes such as AtNHX1, GhNHX1, OsNHX1, and EgNHX1 etc [3,4,6,7]. All these results suggest that vacuolar Na+/H+ antiporter genes play significant roles in salt tolerance
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