Abstract
ABSTRACT In vitro clonal propagation system for Crataeva nurvala, a valuable medicinal tree utilized in traditional systems of Indian medicine, has been developed. Multiple shoot induction was achieved through axillary branching, using stem node segments as explants collected from field grown plants. Cytokinins (6-benzyladenine and kinetin) were investigated for in vitro shoot multiplication and BA proved to be more effective. Formation of callus at the shoot bases, which hindered further growth, was overcome by addition of 30 μM silver nitrate to the medium. A shoot multiplication rate of 3.54-fold was obtained every 4 weeks on MS medium supplemented with 10 μM BA. The MS medium containing 15 μM IAA stimulated significantly higher root initiation (56.66 %) than NAA, IPA, or IBA containing medium. After in vitro hardening in soilrite, the plantlets are growing well in a mixture of sand, soil, and farmyard manure (1:1:1, v/v) in polybags.
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