Abstract
The current research concerns the clinicopathological significance of MHC class I chain-related protein A (MICA) expression in oral squamous cell carcinomas (OSCCs). The expression and location of MICA protein in 14 normal oral mucous and 45 cancerous and para-cancerous tissues were assessed by immunohistochemistry and levels of MICA mRNA expression in 29 cancerous and para-cancerous tissues were determined by the real-time polymerase chain reaction. Data were analyzed with the SPSS16.0 software package. MICA was found to be located in the cytoplasm and plasma membrane. Expression was higher in para-cancerous than in cancerous tissues (P<0.05). However, no statistical difference was found between the following: 1) para-cancerous tissue with normal mucosa; 2) normal mucosa with cancerous tissue;and 3) among different clinicopathological parameters in OSCC (P>0.05). The level of MICA mRNA was higher in OSCCs than in para-cancerous tissues, and was correlated with the regional lymph node status and disease stage (P<0.05). The levels of MICA protein and mRNA expression differ among normal oral mucosa, para-cancerous tissue, and cancerous tissue. MICA may contribute to the tumorigenesis and progression of OSCC.
Highlights
Introduction on Oral squamous cell carcinoma (OSCC) immunotherapyMHC-I type chain-related protein A (MICA) is a newly discovered transmembrane glycoprotein encoded by MHC genes
The expression and location of MICA protein in 14 normal oral mucous and 45 cancerous and para-cancerous tissues were assessed by immunohistochemistry and levels of MICA mRNA expression in 29 cancerous and para-cancerous tissues were determined by the realtime polymerase chain reaction
The primers were as MICA protein expression and localization MICA was mainly expressed in the spinous layer in normal oral mucosa and para-cancerous tissues
Summary
Introduction on OSCC immunotherapyMHC-I type chain-related protein A (MICA) is a newly discovered transmembrane glycoprotein encoded by MHC genes. MICA mainly functions as natural killer (NK) cells, cytotoxic T lymphocytes (CTLs), and other immune effector cell activation receptor NKG2D ligands. These ligands can bind NKG2D-specific proteins, transmit activation signals, induce immune effector cells to clear rapidly the tumor cells of abnormal ligand expression, and play roles in immune surveillance (Bauer et al, 1999; Wu et al, 1999; Li et al, 2001). Oral squamous cell carcinoma (OSCC) is a common malignancy of the head and neck, and knowledge on the effect of MICA expression on this cancer is limited. We used immunohistochemistry and real-time quantitative polymerase chain reaction (PCR) to explore MICA protein and mRNA content in OSCCs. The relationship between clinical and pathological parameters was analyzed.
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