Clinically relevance of ectopic bone formation to the structure of TGF- β superfamily in stress condition

Abstract

Formation of bone outside the skeletal system which can occur in all kinds of soft tissues is called Ectopic or Heterotopic Ossification (HO). It is the abnormal formation of true bone within extra skeletal soft tissues. The TGF-β super family several physiological processes are mediate by these polypeptides such as regulates bone formation and resorption as well as osteoclasts differentiation and survival is stimulated by them. Total of 21 head injured patients admitted in the hospital between 8 to 33 years old. All individuals are treated with indomethacin to control HO formation. The red light treatment and heat lamp exposure were done for 20 min in each day of physiotherapy. Both effective members of transforming growth factor- β, superfamily were analyzed by molecular mechanic methods in amber force field. Montecarlo simulation was done for 200 pSec and the ψ, φ angles of each amino acids were calculated. Clinically, of 25 potentially relevant records identified, 21 were related to HO. Following the treatment, 11 individuals showed the response to heat exposure and physical exercises. 9 out of 21 not only never do effective response to heat treatment, but also progress the HO formation. The results reveal that 2 tgi tends to arrange the protein critical angels of φ and ψ, into more favored and/ or allowed region and the formation and development of right handed, left handed α- helices and β- pleated sheath will be more facilitated because of rising in temperature. It is suggested that this phenomenon cause 2tgi to act in a better thermodynamic situation. Key words: Ramachandran plot, Montecarlo simulation ectopic bone formation, transforming growth factor- β; 1tfg; 2tgi.