Abstract
Simple SummaryThe reactive oxidative metabolites (ROMs), the antioxidant barrier (OXY), the ratio between ROMs and OXY (OSi), thiol groups of plasma compounds (SHp) and the concentration of High Mobility Group Box-1 protein (HMGB-1) were evaluated in dogs naturally infected by Leishmania spp., correlating them with hematological and biochemical parameters. Results showed a significant increase in SHp levels, and a negative correlation between ROMs and the number of red blood cells, and between HGMB-1 and SHp, suggesting the potential role of oxidative stress in the pathogenesis of canine leishmaniosis.This study aimed to investigate the role of oxidative stress parameters (ROMs, OXY, SHp), the Oxidative Stress index (OSi), and High Mobility Group Box-1 protein (HMGB-1) in canine leishmaniosis (CanL). For this study, thirty dogs, naturally infected with Leishmania spp. (Leishmania Group, LEISH) and ten healthy adult dogs (control group, CTR) were included. The diagnosis of CanL was performed by a cytological examination of lymph nodes, real time polymerase chain reaction on biological tissues (lymph nodes and whole blood), and an immunofluorescence antibody test (IFAT) for the detection of anti-Leishmania antibodies associated with clinical signs such as dermatitis, lymphadenopathy, onychogryphosis, weight loss, cachexia, lameness, conjunctivitis, epistaxis, and hepatosplenomegaly. The HMGB-1 and oxidative stress parameters of the LEISH Group were compared with the values recorded in the CTR group (Mann Whitney Test, p < 0.05). Spearman rank correlation was applied to evaluate the correlation between the HMGB-1, oxidative stress biomarkers, hematological and biochemical parameters in the LEISH Group. Results showed statistically significant higher values of SHp in the LEISH Group. Specific correlation between the ROMs and the number of red blood cells, and between HGMB-1 and SHp were recorded. These preliminary data may suggest the potential role of oxidative stress in the pathogenesis of CanL. Further studies are undoubtedly required to evaluate the direct correlation between inflammation parameters with the different stages of CanL. Similarly, further research should investigate the role of ROMs in the onset of anemia.
Highlights
The Canine leishmaniasis (CanL), caused by Leishmania infantum, is a vector-borne zoonotic protozoan disease transmitted by the bite of a female phlebotomine sand fly
The present study aimed to evaluate the reactive oxidative metabolites (ROMs), the antioxidant barrier (OXY), the ratio between ROMs and OXY (OSi), thiol groups of plasma compounds (SHp) and the concentration of High Mobility Group Box-1 protein (HGMB-1) in blood samples of dogs naturally infected with Leishmania spp
This study aimed to evaluate the possible changes in the oxidative stress biomarkers and HGMB-1 in dogs affected by CanL and to correlate these values with hematological and biochemical parameters
Summary
The Canine leishmaniasis (CanL), caused by Leishmania infantum, is a vector-borne zoonotic protozoan disease transmitted by the bite of a female phlebotomine sand fly. Leishmania infantum is the same causative agent of the Zoonotic Visceral Leishmaniasis (ZVL), a disease endemic in the region of the Mediterranean basin, Africa, Asia, and South America, affecting more than 100,000 people annually [1,2]. The dog is considered the main reservoir of the parasite and plays an important role in the transmission of the disease to humans [3]. The appearance of a broad spectrum of clinical signs (cutaneous alterations, lymphadenomegaly, onychogryphosis) and/or pathological abnormalities such as glomerulonephritis, polyarthritis, or uveitis derived from immune-complexes deposition [11,12,13] are considered the consequence of the immunological imbalance
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