Abstract
The aim of this study was to investigate KAI1/CD82 protein expression in human nasopharyngeal carcinoma (NPC) cell lines and human NPC tissues. Immunohistochemistry and western blot analysis were used to detect the localization and expression levels of the KAI1/CD82 protein in five human NPC cell lines. Immunohistochemistry was also conducted to detect the expression of the KAI1/CD82 protein in 70 NPC tissues and 30 non-neoplastic nasopharyngeal tissues. The levels of KAI1/CD82 protein expression were found to decrease as the metastatic potential of cells increased. The expression rate of KAI1/CD82 protein in the NPC tissues (44.3%) was significantly lower than that in the non-neoplastic nasopharyngeal tissues (70.0%) (P<0.05). KAI1/CD82 protein expression in the NPC tissues was not associated with clinical parameters, including gender, age, histological type and T stage, and the positive expression of KAI1/CD82 decreased with increased N staging. The level of KAI1/CD82 protein expression was increased in different human NPC cell lines. The KAI1/CD82 gene was highly expressed in cells with low metastatic potential, while low expression was observed in cells with a high metastatic potential. In addition, the KAI1/CD82 gene was expressed at low levels in nasopharyngeal carcinoma tissues, while high expression was identified in non-neoplastic nasopharyngeal tissues, and was associated with lymph node metastasis. These results indicated that the KAI1/CD82 gene may be involved in the occurrence, development and metastasis of nasopharyngeal carcinoma.
Highlights
Nasopharyngeal carcinoma (NPC) is a highly invasive malignant tumor, which occurs in the nasopharynx
Immunohistochemistry and western blot analysis were used to detect the levels of KAI1/CD82 protein expression in five different human NPC cell lines, CNE‐1, CNE‐2Z, SUNE‐1, SUNE‐1‐5‐8F and SUNE ‐1‐6‐10B, and immunohistochemistry was performed to detect the KAI1/CD82 protein expression in NPC and non‐neoplastic nasopharyngeal tissues
The immunohistochemical results showed that the KAI1/CD82 protein was significantly expressed in the membrane and/or cytoplasm of all five NPC cell lines, with the positive signal of brown particles
Summary
Nasopharyngeal carcinoma (NPC) is a highly invasive malignant tumor, which occurs in the nasopharynx. It is rare in the majority of countries [1], the incidence and mortality rates of NPC are markedly high among Southern Chinese populations. The inhibitory effects of the TM4SF protein on tumor metastasis have been demonstrated [4]; its cell‐membrane location and extensive glycosylation leads to cell‐cell and cell‐extracellular matrix interactions, which subsequently affect tumor metastasis. These interactions are extremely important with regard to the invasion and metastasis of tumors. The aim of this study was to investigate novel methods, which may improve the treatment efficacy and the prognosis, as well as reduce the occurrence of metastasis
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
