Abstract
BackgroundAstrocyte elevated gene-1 (AEG-1) is related to the tumorigenesis and deterioration of different cancers, including non-small cell lung cancer (NSCLC). However, the effect of AEG-1 in NSCLC remains unclear. In this study, we aimed to investigate the clinical significance and effect of AEG-1 on biological function of NSCLC.ResultsAEG-1 was significantly overexpressed in NSCLC tissues and closely correlated to the deterioration of NSCLC based on tissue microarray, TCGA database and meta-analysis. After knock-down of AEG-1, the proliferation, migration and invasion of NSCLC cells were all inhibited, and the tumorigenic and angiogenic ability of NSCLC cells were weakened. Furthermore, the AEG-1 co-expressed genes were significantly related to AMPK signaling pathway based on bioinformatics approaches.Materials and MethodsA tissue microarray, the Cancer Genome Atlas (TCGA) database, as well as a meta-analysis were performed to analyze the relationship between AEG-1 and the clinicopathological parameters of NSCLC. Furthermore, immunocytochemistry, Western blot analysis, scratch assay, colony formation assay, Transwell migration and invasion assay and the chick embryo chorioallantoic membrane (CAM) model were conducted to explore the effect of AEG-1 on NSCLC in vitro and in vivo. Additionally, bioinformatics analyses were carried out to assess the potential pathways and networks of the co-expressed genes of AEG-1.ConclusionsAEG-1 is positively activated in the tumorigenesis and deterioration of NSCLC. We hypothesize that AEG-1 could play an important role in NSCLC via AMPK signaling pathway. Inhibiting the expression of AEG-1 is expected to become a novel method in the therapeutic strategies of NSCLC.
Highlights
Lung cancer has become the most common cancer, and the incidence rate of lung cancer is gradually growing [1,2,3,4]
Astrocyte elevated gene-1 (AEG-1) was significantly overexpressed in non-small cell lung cancer (NSCLC) tissues and closely correlated to the deterioration of NSCLC based on tissue microarray, the Cancer Genome Atlas (TCGA) database and meta-analysis
AEG-1 is positively activated in the tumorigenesis and deterioration of NSCLC
Summary
Lung cancer has become the most common cancer, and the incidence rate of lung cancer is gradually growing [1,2,3,4]. Growing evidence reveals that AEG-1 is significantly related to the invasion, migration, apoptosis and prognosis of different cancers, such as hepatocellular carcinoma [9], bladder cancer [10], prostate cancer [11], gastric cancer [12] and so on. Sun et al [14] reported that the high AEG-1 expression was positively related to carcinogenesis and aggressiveness of NSCLC. Yao et al [15] showed that a low AEG-1 expression was correlated with the clinical stage and lymph node metastasis of NSCLC and could provide potential in predicting metastasis and prognosis of NSCLC patients. Astrocyte elevated gene-1 (AEG-1) is related to the tumorigenesis and deterioration of different cancers, including non-small cell lung cancer (NSCLC). We aimed to investigate the clinical significance and effect of AEG-1 on biological function of NSCLC
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