Clinical, haematological and blood biochemical parameters in Arabian one-humped camels (Camelus dromedarius) with Babesia caballi infection

Abstract

This study was conducted to diagnose Babesia caballi in one-humped camels in Nineveh governorate, Iraq, using microscopic examination and conventional polymerase chain reaction (PCR) and to evaluate the clinical, haematological and blood biochemical parameters in camels. A total of 62 camels from local breeds, 2-7 years old from both sexes, reared in Nineveh Governorate, were naturally infected with B. caballi and 12 clinically healthy camels were used as controls based on clinical and laboratory examination. Babesia caballi was diagnosed as pear-shaped organisms, existing mainly in pairs within the erythrocytes in blood smears stained by Giemsa, with mean parasitaemia rate 8.59±0.13%. Blood smears stained by acridine orange and conventional PCR confirmed B. caballi diagnosis. Infected camels were found to be suffering from partial or total loss of appetite, emaciation, pale mucous membranes, irregular heart beats, rough hair coat, digestive disturbances, haemoglobinuria and presence of ticks on various body parts. There was considerably elevated body temperature, respiratory and heart rates among infected camels in comparison with control groups. Haematological examinations indicated a statistically significant (P<0.05) decrease in red blood cell counts, haemoglobin and haematocrit with macrocytic hypochromic anaemia. Additionally, leukocytosis due to significant (P<0.05) rise in total leukocyte count and lymphocytes in infected camels in comparison with controls besides a significant (P<0.05) decrease in platelet count and fibrinogen were demonstrated. On the other hand, the mean platelet volume, platelet distribution width, clotting time, prothrombin time and activated partial thromboplastin time were significantly (P<0.05) elevated in infected camels vs control. Biochemical analysis revealed significant (P<0.05) rise in troponin I, creatine phosphokinase myocardial band and lactate dehydrogenase in infected camels compared to healthy animals. This study concluded that B. caballi infected camels might be endemic in Nineveh governorate, Iraq. Babesia caballi in camels causes clinical, haematological, and blood biochemical changes.