Abstract
BackgroundMicroRNAs play a vital role in coronary artery disease. Abnormal expression of microRNAs has been found to be associated with the occurrence of CAD.MethodsWe identified significantly differentially expressed microRNAs in plasma between 40 patients with CAD and 10 controls with NCA using RNA sequencing. The differentially expressed microRNAs were analyzed for Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment.ResultsFifty cDNA libraries were constructed and sequenced, and a total of 1871.82 M raw reads were obtained, and 2135 microRNAs were found. Compared to the expressed microRNAs of NCA controls, 159 microRNAs were differentially expressed in CAD patients, including 119 upregulated microRNAs and 40 downregulated microRNAs. The top 10 upregulated miRNAs were miR‐144‐3p, miR‐34a‐5p, miR‐15b‐3p, miR‐22‐3p, miR‐29b‐3p, miR‐1270, miR‐6891‐5p, miR‐106a‐5p, miR‐15b‐5p, and hsa‐miR‐499b‐3p. The top ten downregulated miRNAs were miR‐4437, miR‐6842‐3p, miR‐4664‐3p, miR‐671‐3p, miR‐219a‐1‐3p, miR‐7848‐3p, miR‐664a‐3p, miR‐1284, miR‐361‐3p, and miR‐6780a‐5p. The target genes of differentially expressed microRNAs were related to many basic biological terms, such as biological process, cellular component, and molecular function. According to the KEGG pathway analysis, the most enriched pathways of the differentially expressed microRNAs were endocytosis, focal adhesion, axon guidance, and so on. Furthermore, six upregulated and two downregulated microRNAs were detected by qRT‐PCR (Quantitative Real‐time PCR) and ROC analysis for diagnosing CAD.ConclusionThe results suggest that the expression levels of some microRNAs may play a vital role in the physiological and pathological course of CAD. Our study may provide useful information for the diagnosis and treatment of CAD.
Highlights
Coronary artery disease (CAD) is a common heart disease
acute coronary syndrome (ACS) can be divided into unstable angina (UA), acute non‐ST‐segment eleva‐ tion myocardial infarction (NSTEMI) and acute ST‐segment elevation myocardial infarction (STEMI), according to severity
STEMI and NSTEMI are together known as acute myocardial infarction (AMI).[4,5]
Summary
Coronary artery disease (CAD) is a common heart disease. It is a myocardial malfunction and structural lesion caused by coronary artery stenosis and poor blood supply. Inflammation is involved in various stages of the formation and development of atherogenesis plaque, and inflamma‐ tory factors produced in this process are predicted to be potential markers of CAD They play a key role in coordinating the interac‐ tions of the cells during the development of atherogenesis.[8,9,10,11] Inflammatory mediators involved in the function of some cells, in‐ cluding endothelial cells, macrophages, and T cells. They stimulate some signalling pathways in the onset and progression of atheroscle‐ rosis.[12,13] They can be regulated by microRNAs (miRNAs), which are small non‐coding RNAs that have emerged as important regulators in atherosclerosis.[14,15,16,17,18]. We explored the role of microRNAs in CAD, and we further explored the relationship between microRNAs and the onset and progression of CAD to provide new ideas for the diagnosis and treatment of CAD
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