Abstract
Accumulated evidences suggested that circular RNAs (circRNA) played critical roles in tumorigenesis and progression. To our knowledge, no study reported the function of circular RNA DGKB (circDGKB, circRNA ID: hsa_circ_0133622) on progression of neuroblastoma (NB). Here, we showed that circDGKB was upregulated in NB tissues compared to the normal dorsal root ganglia. Moreover, the expression level of circDGKB was negatively correlated with the survival rate of NB patients. Mechanically, overexpression of circDGKB promoted the proliferation, migration, invasion, and tumorigenesis of NB cells and reduced cell apoptosis, and vice versa. In addition, qRT-PCR and/or Western blot results showed that circDGKB overexpression inhibited the expression level of miR-873 and enhanced GLI1 expression. Moreover, miR-873 functioned an opposite role to circDGKB and significantly weakened circDGKB role in promoting NB progression. Furthermore, GLI1 upregulation also rescued the miR-873 role in inhibiting NB progression. In conclusion, our work proved that circDGKB promoted NB progression via targeting miR-873/GLI1 axis in vitro and in vivo. Our study provided a new target for NB treatment and indicated that circDGKB could act as a novel diagnostic marker for NB.
Highlights
Neuroblastoma (NB) is a malignant tumor mainly occurring in children [1], which commonly arises in adrenal glands, and occurs in the abdomen, neck, spine, or chest [2]
We found that circDGKB was highly expressed in NB
We found that circDGKB downregulation could lead to S phase reduction (Figures 2G,H)
Summary
Neuroblastoma (NB) is a malignant tumor mainly occurring in children [1], which commonly arises in adrenal glands, and occurs in the abdomen, neck, spine, or chest [2]. Patients with low-risk disease respond positively to therapy and even can recover without any treatment. Numerous studies demonstrated that circRNA/miRNA axis played critical roles in the progression of cancers. Mao et al [11] demonstrated that circ0068871 could accelerate the progression of bladder cancer by targeting miR-181a-5p and regulate the expression of FGFR3 and activate STAT3. Huang et al [12] identified that circ100338 was highly expressed in hepatocellular carcinoma (HCC) and functioned as an inhibitor of miR-141-3p to promote the proliferation of HCC cells. MiRNAs were proved to participate in progress of many kinds of cancers by acting as the targets of circRNAs and regulating the expression of oncogenes or tumor suppressive genes [15]. We found that circDGKB (circRNA ID: hsa_circ_0133622) was highly expressed in NB. Our study provided a novel potential target for the diagnosis and treatment of NB in clinical
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