Abstract
As a malignant tumor of the central nervous system, glioma exhibits high incidence and poor prognosis. Circular RNA HIPK3 (circHIPK3) is a circular RNA (circRNA) related to cancer progression. However, the role of circHIPK3 in gliomas remains unclear. The purpose of this study was to investigate the role of circHIPK3 in gliomas and its mechanism. The qRT-PCR method was used to determine the expression pattern of circHIPK3 in glioma cell lines. CCK-8 assay was used to detect cell proliferation. Cell migration and invasion were evaluated using the Transwell assay. Our results showed that circHIPK3 expression was significantly up-regulated in glioma tissues and cell lines. In vitro, the down-regulation of circHIPK3 significantly inhibited the proliferation, migration and invasion of glioma cells. Besides, we demonstrated that circHIPK3 acted as a sponge to absorb miR-124 and promoted CCND2 expression. In summary, our results indicated that circHIPK3 had carcinogenic effects by regulating the expression of CCND2 in glioma by sponging miR-124. These findings provided favorable evidence to reveal the role of circHIPK3 in the development of gliomas.
Highlights
Glioma is the most severe primary human central nervous tumor in adults worldwide, accounting for 40% of intracranial tumors, and has a poor prognosis (Geng et al, 2015)
The growth and metastasis of glioma cells depend on angiogenesis, and a continuous increase in blood vessels has been considered a key feature of gliomas (Lund et al, 1998)
We found that circHIPK3 interacted with 20 RNA Binding Protein (RBP), including AGO1, AGO2, AGO3, CAPRIN1, EIF4A3, ELAVL1, FMR1, FUS, FXR1, FXR2, IGF2BP1, IGF2BP2, IGF2BP3, LIN28A, LIN28A, LIN28B, MOV10, PTBP1, U2AF2, UPF1
Summary
Glioma is the most severe primary human central nervous tumor in adults worldwide, accounting for 40% of intracranial tumors, and has a poor prognosis (Geng et al, 2015). The growth and metastasis of glioma cells depend on angiogenesis, and a continuous increase in blood vessels has been considered a key feature of gliomas (Lund et al, 1998). This characteristic of rapid growth and high infiltration causes most patients already at stage IV when they are diagnosed with glioma (Shraddha et al, 2015). Pathological diagnosis is the basis for treatment. When considering the prognosis of osteosarcoma, a biopsy is performed to obtain confirmation by pathological examination as soon as possible, which is of great significance for the diagnosis and treatment. In order to overcome the existing challenges, efforts need to be focused on developing new therapies for gliomas
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
