Circadian patterns of breathing.

Abstract

In the rat, a mostly nocturnal animal, activity, body temperature and metabolic rate increase during the dark hours of the day. Since all these variables are known to influence breathing, it is expected that also pulmonary ventilation (VE) will present a circadian pattern. In rats chronically instrumented for measurements of body temperature and activity by telemetry, carbon dioxide production and oxygen consumption (V(O(2))) were measured continuously for several days by an open-circuit method, while VE was monitored by a modification of the barometric technique. Tidal volume, frequency, and VE increased in the dark (D) compared to the light (L) hours, with minor L-D differences in VE/V(O(2)). L-D differences in activity were not responsible for the circadian pattern of VE. Both in hypoxia and in hypercapnia the degree of hyperventilation (percent increase in VE/V(O(2))) was essentially independent of the time of the day, despite the fact that in hypoxia, differently from hypercapnia, the amplitude of the circadian pattern of all variables decreased, activity being the least affected, and body temperature the most. These effects of hypoxia, which occurred before and after sino-aortic denervation and did not compromise the period of the biological clock, may be mediated by the hypothalamic thermoregulatory centers. The data of these experiments and of others reviewed in this article indicate that (1) breathing and its control mechanisms accompany the daily oscillations of numerous physiological variables, and (2) the advantages of a biological clock do not compromise the adequacy of the hyperventilatory responses to chemical challenges.