Cibacron Blue F3GA functionalized poly(vinyl alcohol- co-ethylene) (PVA- co-PE) nanofibrous membranes as high efficient affinity adsorption materials

Abstract

Poly(vinyl alcohol- co-ethylene) (PVA- co-PE) nanofibrous membranes with surface incorporated Cibacron Blue F3GA (CB) were prepared for bio-separation and purification applications. PVA- co-PE nanofibers were fabricated via melt extrusion of an immiscible blend of PVA- co-PE polymer with cellulose acetate butyrate (CAB) as a sacrificial matrix and subsequent removal of CAB. PVA- co-PE nanofiber dispersions were coated on a releasing surface to form nanofibrous membranes with fiber sizes ranging in 50–300 nm. Cibacron Blue F3GA (CB) was covalently immobilized onto the nanofiber surfaces and reached a maximum amount of 220 mg/g on the nanofibers. The CB attached PVA- co-PE nanofibrous membranes could adsorb large amount of bovine serum albumin (BSA) (105.5 mg/g nanofiber) at pH 5.0 while the non-specific BSA adsorption on the pristine PVA- co-PE nanofibrous membrane was negligible. The elution efficiency of BSA in 1 M NaSCN was 92.5%. After 10 adsorption–elution cycles, a slight decrease of BSA capture capacity on the membrane was observed. This work demonstrated the feasibility of using the CB functionalized PVA- co-PE nanofibrous membranes in bio-separation and purification applications.