Chronic high fat diet decreases CD34/CD133 cell population in bone marrow and peripheral circulation in association with decreased level of serum MG53

Abstract

Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play an important role in maintaining the function and integrity of vasculature. The number of EPCs is significantly decreased in hyperlipidemic animals and patients. The present study was to determine if chronic high fat diet (HFD) treatment could modify the the EPC population in BM and blood and the relationship to the membrane protective protein MG53 and reactive oxygen species (ROS). EPC numbers and ROS formation in BM and blood were determined with flow cytometry in male C57BL/6 mice treated with HFD for 6 months. Serum MG53 level was determined using Western blot. Mice on normal diet were used as control. Chronic HFD treatment significantly increased the blood cholesterol and glucose levels. Surprisingly, HFD treatment did not significantly change the intracellular and extracellular ROS production in BM and blood. In addition, HFD treatment significantly suppressed the serum MG53 level. HFD also significantly decreased the number of CD34/CD133 double positive EPCs in both BM and blood. Taken together, chronic HFD treatment significantly decreased the population of CD34/CD133 double positive EPCs in both BM and blood in association with decreased serum MG53 but had no effect on intracellular and extracellular ROS production in BM and blood. These results suggest a potential role of circulating MG53 for the maintenance of EPCs population and integrity of vasculature.