Abstract
We have previously demonstrated that chronic ethanol specifically decreases the hepatic level and rate of synthesis of 2,6‐sialyltrans‐ferase (2,6‐ST). To understand its mechanism of action, effects of 8 weeks of chronic ethanol feeding on the expression of sialyltrans‐ferase (ST) genes in rat liver and kidneys were determined by North‐emblot analysis of ST mRNAs. It was found that, compared with the pair‐fed control rats, the percentage decreases in ST mRNAs in the ethanol‐fed group were as follows: liver—Gal‐β‐1,4GlcNAc α2,6‐ST (2,6‐ST): 59% (p < 0.001); liver—Gal‐β‐1,3GlcNAc α2,3‐ST (2,3‐ST): 32% (p < 0.01); and kidneys—2,6–ST: 5% (NS). In contrast, glyceral‐dehyde‐3‐phosphate dehydrogenase mRNA in both liver and kidneys was unaffected by the same ethanol treatment. Taken together, these results demonstrate that chronic ethanol downregulates the expression of 2,6‐ST and 2,3‐ST genes in rat liver.
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