Chromosome-level genome assembly of the dotted gizzard shad ( Konosirus punctatus) provides insights into its adaptive evolution.

Abstract

<i>Konosirus punctatus</i> is an economically important marine fishery resource and is widely distributed from the Indian to Pacific oceans. It is a good non-model species for genetic studies on salinity and temperature adaptation. However, a high-quality reference genome has not yet been reported. Here, an 800.00 Mb high-quality chromosome-level genome with a contig N50 length of 2.14 Mb was assembled using Illumina, Pacific Biosciences, and Hi-C sequencing technology. The assembled sequences were anchored to 24 pseudochromosomes by the Hi-C data. In total, 24 298 protein-coding genes were predicted, 91.08% of which were successfully annotated with putative functions. Furthermore, 587 putative genes were identified as being under positive selection. This new high-quality <i>K. punctatus</i> reference genome provides a fundamental resource for a deeper understanding of temperature and salinity adaptation and species conservation.