Abstract
BackgroundIn vitro oocyte maturation (IVM) is being increasingly approached in assisted reproductive technology (ART). This study aimed to evaluate the quality of embryos generated by in-vitro matured immature follicles, as a guideline for further clinical decision-making.MethodsA total of 52 couples with normal karyotypes underwent in vitro fertilization, and 162 embryos were donated for genetic screening. Embryos in IVF group were generated by mature follicles retrieved during gonadotrophin-stimulated in vitro fertilization (IVF) cycles. And embryos in IVM group were fertilized from IVM immature oocytes.ResultsThe average age of the women was 30.50 ± 4.55 years (range 21–42 years) with 87 embryos from IVF group and 75 embryos from IVM group. The rate of aneuploid with 28 of the 87 (32.2%) embryos from IVF group and 21 of the 75 (28%) embryos from IVM group, with no significant difference. The frequency of aneuploid embryos was lowest in the youngest age and increased gradually with women’s age, whether in IVF group or IVM group and risen significantly over 35 years old. The embryos with morphological grade 1 have the lowest aneuploidy frequency (16.6%), and increase by the grade, especially in IVF group. In grade 3, embryos in IVM group were more likely to be euploid than IVF group (60% vs 40%, respectively).ConclusionsIVM does not affect the quality of embryos and does not increase the aneuploidy rate of embryos. It is clinically recommended that women more than 35 years have a high aneuploidy rate and recommended to test by PGS (strongly recommended to screened by PGS for women more than 40 years). Women aged less than 35 years old for PGS according to their physical and economic conditions. Embryo with poor quality is also recommended to test by PGS, especially for grade III embryos.
Highlights
In vitro oocyte maturation (IVM) is being increasingly approached in assisted reproductive technol‐ ogy (ART)
Baart et al suggested that high-dose of gonadotrophin-stimulated in controlled ovarian hyperstimulation (COH) cycle may lead to high frequency of aneuploidy [3]
5.7% triploid, 6.9% haploid, 8.0% mosaic, 4.6% fragment abnormality, and 10.3% chaotic in 87 embryos samples of in vitro fertilization (IVF) group vs. only 5.5% triploid, 8.0% haploid, 4.0% mosaic, 4.0% fragment abnormality, and 13.3% chaotic were detected in 75 embryos samples of IVM group (Table 1; Fig. 1)
Summary
In vitro oocyte maturation (IVM) is being increasingly approached in assisted reproductive technol‐ ogy (ART). This study aimed to evaluate the quality of embryos generated by in-vitro matured immature follicles, as a guideline for further clinical decision-making. In vitro fertilization (IVF) cycles are immature [1], and these immature oocytes are usually discarded. Baart et al suggested that high-dose of gonadotrophin-stimulated in COH cycle may lead to high frequency of aneuploidy [3]. In vitro maturation (IVM) of immature follicles as an alternative method which has become a promising strategy to maximize the utilization of follicles and maximum cost savings, it could be beneficial for women with polycystic ovaries, poor ovarian response to gonadotropin treatment, premature ovarian failure, and cryopreserve their oocytes before monotherapy to preserve their fertility [4]. More than half of embryos produced by IVF cycles are aneuploid [6]
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