Abstract
The observed effect of A-methopterin upon Escherichia coli metabolism has been the intracellular accumulation of 5-amino-4-imidazole-carboxamide ribotide, with the concomitant exclusion of formate-C 14 and glycine-C 14 from purine derivatives. Correlation between the observed amount of intracellular accumulation of aminoimidazolecarboxamide ribotide and the degree of growth inhibition by various levels of A-methopterin provided evidence that blockade of the de novo synthesis of purines was the primary site of A-methopterin inhibition. The metabolic sequence from formate to the various purine ribotides was apparently the same for an A-methopterin-resistant strain of E. coli as for its parent strain.
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