Abstract
High-performance liquid chromatography (HPLC), thin-layer chromatography (TLC) and gas—liquid chromatography (GLC) methods are described for separating sucrose monostearate isomers. The HPLC procedure provides baseline separation of purified monoester isomers into three main peaks at room temperature, and completely separates monoesters with different acyl chain lengths (C 14, C 16, C 18). The TLC method separates up to six of the eight possible positional monostearate isomers, which can be further differentiated by specific color development with a visualizing agent. The GLC technique used resolves monoesters with different acyl chain lengths, and partially separates monostearate isomers. Isomers collected from HPLC were subjected to treatment with invertase, then resolved and detected by TLC to determine fatty acid substitution patterns on the sucrose molecule.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
