Abstract
The cultured cells of calli fromAloineae plants were classified into two groups based on the nuclear variations in morphology, cytochemical histone variation, and RNA synthetic activity. The first group (cell types I a, II a, and III) had diffused type chromatin, histones rich in lysine as revealed cytochemically, and were active in RNA synthesis. In contrast, the second group (cell types I b, II b, and IV) revealed condensed type chromatin, histones rich in arginine, and were inactive in RNA synthesis. When cells of the second group were treated with sodium stylenesulfonate (SS), the chromatin structure and its cytochemical nature became similar to those of the first group. Recovery of RNA synthetic activity also appeared.
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