Abstract

Bovine ovaries were perfused in vitro with acetate-1-14C throughout an experiment which lasted 377 minutes. Luteal ovaries and the contralateral follicular ovaries were sequentially infused with prolactin, and then with LH. At the end of the perfusion, the specific activity of luteal cholesterol-14C was slightly greater than the specific activity of either the luteal tissue progesterone-14C or the secreted progesterone-14C. The specific activity of the cholesterol-14C isolated from the residual stromal tissue and from the follicular ovaries was much lower than that observed in the corpus luteum.

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