Abstract
Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs–CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV–visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000–1,1000 and 120–480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.
Highlights
Aminoglycosides (AMGs) are RNA-binding antibiotics sharing a common core structure named the streptamine ring
The sharp peak could be considered as the surface plasmon resonance (SPR) band of AgNPs, which is dependent on the physicochemical properties of the AgNPs, including their particle size, shape, surface chemistry, medium, and ionic strength [15]
We have shown that SPR can be used to analyze various AMG antibiotics
Summary
Aminoglycosides (AMGs) are RNA-binding antibiotics sharing a common core structure named the streptamine ring. Farmers tend to use AMG antibiotics to treat some bacterial infections in agricultural crops and livestock animals [2]. The clinical use of AMG antibiotics has been strictly defined because of its irreversible toxicity to vital organs, ears and kidneys [3]. Most AMG antibiotics are still often and extensively used in animal husbandry owing to their low-cost, causing potential residues in the water and food chain [2]. Test kits and other laboratory-based methods used for the detection of antibiotics are mainly based on microbial inhibition assays. Either cannot detect AMGs or have a much higher limit of detection (LOD) relative to the maximum residue limit [4]. Conventional liquid chromatography–tandem mass spectrometry (LC–MS/MS) offers an effective alternative to these methods [5] because it allows screening, confirmation, and validation of the target analyte
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