Abstract
A pool of chlorophyllide b has been detected in greening cucumber cotyledons. This pool exhibited the same spectrofluorometric properties as that of Chl b in diethyl ether at 298 and 77 K (Rebeiz, C.A., Belanger, F.C., Freyssinet, G. and Saab, D.G. (1980) Biochim. Biophys. Acta 590, 234–247) but had the chromatographic mobility and solubility of a monocarboxylic phorbin. The presence of a free carboxylic group and of a formyl group in this putative chlorophyllide b pool was demonstrated by methylation with diazomethane and by its conversion into chlorophyllide b oxime upon treatment with hydroxylamine. The concentration of this chlorophyllide b pool in greening tissues was in the same range as those of the protochlorophyllide and chlorophyllide a pools. Less than 15% of the chlorophyllide b pool could have arisen from chlorophyllase activity in vitro, as confirmed by the extent of hydrolysis of 14C-labeled Chl b added to the tissue just before extraction.
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